Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations.

"This book will provide individuals without background knowledge in optical physics, electronics, or image processing with many of the basic facts they need to know to understand both the power and limitations of their images."
—Cell Biology Education on the First Edition

Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags.

Written in simple, clear language, the book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. Including new sections on live cell imaging, measurement of protein dynamics, deconvolution, multiphoton microscopy, and superresolution microscopy, Fundamentals of Light Microscopy and Electronic Imaging, Second Edition features the following chapters:

• Fundamentals of Light Microscopy
• Light and Color
• Illuminators, Filters, and the Isolation of Specific Wavelengths
• Lenses and Geometrical Optics
• Diffraction and Interference in Image Formation
• Diffraction and Spatial Resolution
• Phase Contrast Microscopy and Darkfield Microscopy
• Properties of Polarized Light
• Polarization Microscopy
• Differential Interference Contrast (DIC) Microscopy and Modulation Contrast Microscopy
• Fluorescence Microscopy
• Fluorescence Imaging of Dynamic Molecular Processes
• Confocal Laser Scanning Microscopy
• Two-Photon Excitation Fluorescence Microscopy
• Superresolution Imaging
• Imaging Living Cells with the Microscope
• Fundamentals of Digital Imaging
• Digital Imaging Processing

Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags.

The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy.

PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website:

www.wiley.com/go/murphy/lightmicroscopy